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. 2019 Mar 20;20(6):1415. doi: 10.3390/ijms20061415

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ppCMV_pp65 induced more IFN-γ secretion than ppCMV_IE1, as shown by target cell-dependent IFN-γ ELISpot assay. The assay utilized loaded target cells consisting of CD3 negative cells stimulated overnight with 1 μg/µL ppCMV_pp65 or ppCMV_IE1 incubated at an effector: target ratio of 1:1 for 16 h. Results are shown for (A) all donors (ALL), (B) donors with expected response (DER), and (C) donors with unexpected response (DUR). The T-cell fractions of each donor showed similar patterns against target cells loaded with ppCMV_pp65 and ppCMV_IE1. NF: negative fraction (memory); PF: positive fraction (naive)