Table 2.
For methylation-specific PCR.
| Primer Name | Primer Sequence (5′–3′) | Product Size (bp) | Annealing Temperature (°C) |
|---|---|---|---|
| hMLH1-U (F) | TTTTGATGTAGATGTTTTATTAGGGTTGT | 124 | 60 |
| hMLH1-U (R) | ACCACCTCATCATAACTACCCACA | ||
| hMLH1-M (F) | TATATCGTTCGTAGTATTCGTGT | 153 | 60 |
| hMLH1-M (R) | TCCGACCCGAATAAACCCAA | ||
| E-cadherin-U (F) | TGGTTGTAGTTATGTATTTATTTTTAGTGGTGTT | 120 | 60 |
| E-cadherin-U (R) | ACACCAAATACAATCAAATCAAACCAAA | ||
| E-cadherin-M (F) | TGTAGTTACGTATTTATTTTTAGTGGCGTC | 112 | 64 |
| E-cadherin-M (R) | CGAATACGATCGAATCGAACCG | ||
| p16-U (F) | TTATTAGAGGGTGGGGTGGATTGT | 151 | 60 |
| p16-U (R) | CAACCCCAAACCACAACCATAA | ||
| p16-M (F) | TTATTAGAGGGTGGGGCGGATCGC | 150 | 65 |
| p16-M (R) | GACCCCGAACCGCGACCGTAA | ||
| APC-U (F) | GTGTTTTATTGTGGAGTGTGGGTT | 108 | 61 |
| APC-U (R) | CCAATCAACAAACTCCCAACAA | ||
| APC-M (F) | TATTGCGGAGTGCGGGTC | 98 | 63 |
| APC-M (R) | TCGACGAACTCCCGACGA | ||
| Runx3-U (F) | TTATGAGGGGTGGTTGTATGTGGG | 221 | 56 |
| Runx3-U (R) | AAAACAACCAACACAAACACCTC | ||
| Runx3-M (F) | TTACGAGGGGCGGTCGTACGCGGG | 221 | 66 |
| Runx3-M (R) | AAAACGACCGACGCGAACGCCTCC |
U; unmethylated sequence, M; methylated sequence, APC; adenomatous polyposis coli, F; forward, R; reverse.