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Culture of HGFs. (A) Day 7 of primary culture of HGFs (inverted microscope; magnification, ×400). (B) Day 15 of second generation of HGFs (inverted microscope; magnification, ×400). The primary culture of HGFs exhibited clear outlines and large, spherical or elliptic nuclei. Second generation cells were spindle-shaped and fibroblast-like. HGF, human gingival fibroblast.
