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. 2019 Apr 18;10:122. doi: 10.1186/s13287-019-1228-7

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — PAX6-SOX2 differentially regulate OCT4 and BRN2 expression. a Schematic of OCT4-luciferase reporter constructs. PE and DE, proximal and distal enhancer regions, respectively; red bars—qPCR amplifying fragments in the ChIP-PCR. b Significant reduction of OCT4-luciferase activities in H1 hESCs co-transfected with PAX6 expressing vector. Histograms: quantitative analysis (n = 3) and *p < 0.05; representative immunoblot of PAX6 overexpression in the inserted images. c Schematic of BRN2-enhancer (BRN2-enh) luciferase reporter constructs. d BRN2-luciferase reporter assay in hNPCs. e Effect of SOX2-knockdown (SOX2-KD) and PAX6 overexpression (PAX-OE) on BRN2-enh luciferase activities in hNPCs. ***p < 0.0005 (n = 3)