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. 2019 Mar 14;19(5):3743–3755. doi: 10.3892/mmr.2019.10037

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Copyright: © Ni et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — β-estradiol supplement suppresses the function of gap junctions in Con A stimulated peripheral blood lymphocytes. (A) Control experiments of DiIC₁₈ or calcein AM single-stained cells and DiIC₁₈-calcein double-stained cells were performed in parallel; DiIC₁₈⁺-calcein⁺-positive fluorescent cells expressed as a represent percentage of the total number of peripheral blood lymphocytes. (B) Mean percentages of DiIC₁₈⁺-calcein⁺-double-positive cells of three independent experiments ± standard error from five samples/group. **P<0.01, control group vs. Con A-stimulated group; ^#P<0.05, Con A-stimulated group vs. E-treated group. Con A, concanavalin A; calcein AM, calcein acetoxymethyl ester; E, β-estradiol.