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. 2019 Apr 18;10:123. doi: 10.1186/s13287-019-1221-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — IL-33 protects HSCs from apoptosis after irradiation. a The percentage of annexin⁺ bone marrow Lin⁻c-Kit⁺ cells at 72 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. b The percentage of annexin⁺CD45⁺ cells in the bone marrow at day 7 after 600-cGy TBI and treatment with PBS or IL-33. Data are mean ± SEM (n = 6 in each group). *P < 0.05 compared to untreated controls. c Left, P53 mRNA expression in bone marrow Lin⁻c-Kit⁺ cells at 6 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Right, mean percentages of P53 protein expression in Lin⁻c-Kit⁺ cells at 36 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. d The percentage of annexin⁺ Lin⁻c-Kit⁺ cells with or without P53 inhibition at 72 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. e Left, PUMA mRNA expression in bone marrow Lin⁻c-Kit⁺ cells at 6 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Right, mean percentages of PUMA protein expression in Lin⁻c-Kit⁺ cells at 36 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. f The percentage of annexin⁺ Lin⁻c-Kit⁺ cells with or without PUMA knockdown at 72 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. g CFCs from bone marrow Lin⁻c-Kit⁺ cells with or without PUMA knockdown in irradiated TSF culture and irradiated TSF plus IL-33 culture groups. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant