Table 2.

Advantages and disadvantages of several single cell-analytic methods

Single cell analysis technology	Advantages	Disadvantages	References
Single-cell mass cytometry	Detect multiple protein markers simultaneously	Providing only a coarse level of resolution	[92, 93]
Single-cell RNA sequencing	Characterize the transcriptomes of cell types in different tissues and identifying their transcriptional signatures	Initial amounts of RNA obtained from a single cell are low Exhibiting higher levels of noise and more zero values	[94, 96]
Single-cell proteomics	Capture proteomic information from individual cells Rich in information	Multicellular Single-cell proteomics with high sensitivity, specificity and low cost needs to be developed	[97–99]