FA	Folic acid
THF	tetrahydrofolate
DHFR	dihydrofolate reductase enzyme
hDHFR	human DHFR
bDHFR	bovine DHFR
rhDHFR	recombinant human DHFR
hTS	human thymidylate synthase
NADPH	nicotinamide adenine dinucleotide phosphate
dUMP	dihydrofolate synthase (DHFS), deoxyuridine monophosphate
dTMP	deoxythymidine monophosphate
5,10-Methylene THF	N5-N10-methylenetetrahydrofolate
TrxR	thioredoxin reductases
FRs	folate receptors
ct-DNA	calf thymus-DNA
RNR	ribonucleotide reductase
TS	thymidylate synthase
TP	thymidylated phosphorylase
MTX	methotrexate
ANN	artificial neural network
PDB	Protein Data Bank
Asp127	Aspartate 127
Glu30	Glutamate 30
Phe31	Phenylalanine
ELISA assay	enzyme-linked immunosorbent assay
FDA	U.S. Food and Drug Administration
EMA	European Medicines Agency
ADMET	absorption, distribution, metabolism, excretion, toxicity
OVCAR-3	ovarian cancer cell line
MDA-MB-435	melanoma cell line
NSCLC	non-small-cell lung carcinoma
HCC	hepatocellular carcinoma
A549	adenocarcinomic human alveolar basal epithelial cells
SK-OV-3	human ovary cancer cell line
HCT15; HCT116	human colon adenocarcinoma colorectal adenocarcinoma
K562	human chronic myeloid leukemia cell
HeLa	human of immortal cervical cancer cell
KB	keratin-forming tumor cell line HeLa
MCF-7; MDA-MB-231; MDA-MB-468	human breast cancer cell line
DU145	human prostate cancer cell line
HMEC	normal human mammary epithelial cells
HL-60	human caucasian promyelocytic leukemia cell line
NCI-H1299; NCI-H522	human non-small cell lung carcinoma cell line
HepG2	human liver cancer cell line
SK-n-SH	human neuroblastoma
A17	amacrine cells in mammalian retina
SAR	structure activity relationships