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. 2019 Mar 26;11(3):704. doi: 10.3390/nu11030704

Figure 4.

Figure 4

Expression of TNF-β receptor in three CRC cell lines and resveratrol suppressing their expression. (A) CRC cells in monolayer cultures were immunolabeled with primary antibodies for TNF-β and TNF-β receptor (TNF-βR), followed by incubation with rhodamine-coupled secondary antibodies and counterstaining with DAPI to visualize cell nuclei. Original magnification, 400x. Scale bar = 30 nm. (B) CRC cell lines in monolayer cultures were untreated (Co) or treated, as described above. Whole cell lysates were fractionated and immunoblotted with antibodies against TNF-βR and β-actin. β-actin was used as a loading control in all experiments.