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. 2019 Mar 8;17(3):163. doi: 10.3390/md17030163

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Growth of RBM28-, RPAP3-, and MIA30-knockdown DU145 cells under normoxic and hypoxic conditions. (a) The expression levels of the indicated proteins in each knockdown cell line were analyzed by western blotting. The expression level of each protein was calculated from its relative density, which was normalized to that of β-actin. (b) The proliferation rates of MIA3-, RBM28-, and RPAP3-knockdown DU145 cells were investigated under normoxic and hypoxic conditions. The growth inhibition rate was calculated as a percentage relative to the negative control cells. Differences were considered significant at * p < 0.01.