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. 2019 Apr 17;38:168. doi: 10.1186/s13046-019-1147-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Functional analysis of CD166.BBζ CAR-T cells in vitro. a. Cytotoxic activity of CD166.BBζ CAR-T cells. We used the LDH release assay to evaluate the cytotoxic activity of CD166.BBζ CAR-T cells and non-transduced T cells at different E/T ratios (effector/ target cells). b. Th1/ Th2 cytokine release of CD166.BBζ CAR-T cells. Culture supernatant was collected 24 h later, and the production of IL-2, TNF-α, IFN-γ, IL-4, IL-6, IL-10 were measured using a CBA assay. The results are presented as the mean ± SD from experiments that were performed in triplicate (*P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001)