Fig. 6.

Schematic diagram of optimized C. glutamicum CGMCC 1.15647 for the secretory production of XynA. The chromosomal integrated xynA, plasmids pXMJ19-xynA and pEC-XK99E-xynA contributed to the XynA production by using the native AH6 and cspB2 promoters, and cspB2 signal peptide. In the ClpS and CspB disrupted mutant, protease could make little influence on the recombinant XynA and XynA secretion could encounter less S-layer barrier after the XynA transporting across the membrane by the Sec machine