Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Sources |
|---|---|---|
| Strains | ||
| E. coli DH5α | Lab stock | |
| C. glutamicum ATCC13032 | Wild type | Lab stock |
| C. glutamicum CGMCCl.15647 | Wild type | Lab stock |
| ΔcspB2 | C. glutamicum CGMCCl.15647, cspB2 disruption | This work |
| ΔclpS | C. glutamicum CGMCCl.15647, clpS disruption | Lab stock |
| ΔcspB2ΔclpS | C. glutamicum CGMCCl.15647, cspB2 and clpS disruption | This work |
| ΔcspB2InX | C. glutamicum CGMCCl.15647, cspB2 disruption, xynA integration | This work |
| ΔcspB2ΔclpSInX | C. glutamicum CGMCCl.15647, cspB2 disruption, clpS disruption, xynA integration | This work |
| Cg47+P19-0 | C. glutamicum CGMCCl.15647 harboring pXMJ19 | This work |
| Cg47+P19-X | C. glutamicum CGMCCl.15647 harboring pXMJ19-xynA | This work |
| Cg47+P19-cspB1X | C. glutamicum CGMCCl.15647 harboring pXMJ19-cspB1-cspA-xynA | This work |
| Cg32+P19-X | C. glutamicum ATCC13032 harboring pXMJ19-xynA | This work |
| ΔcspB2+P19-X | ΔcspB2 harboring pXMJ19-xynA | This work |
| ΔclpS+P19-X | ΔclpS harboring pXMJ19-xynA | This work |
| ΔcspB2ΔclpS+P19-X | ΔcspB2ΔclpS harboring pXMJ19-xynA | This work |
| ΔcspB2InX+P19-X | ΔcspB2InX harboring pXMJ19-xynA | This work |
| ΔcspB2ΔclpSInX+P19-X | ΔcspB2ΔclpSInX harboring pXMJ19-xynA | This work |
| ΔcspB2ΔclpSInX+P19-X+pEC-X | ΔcspB2ΔclpSInX harboring pXMJ19-xynA and pEC-XK99E-xynA | This work |
| Plasmids | ||
| pXMJ19 (P19-0) | E. coli–C. glutamicum shuttle vector, Chlr | Lab stock |
| pXMJ19-cspB2 (P19-cspB2) | pXMJ19 derivative, PcspB2, cspB2 signal peptide, cspB2 gene | |
| pXMJ19-cspB1-cspA-xynA (P19-cspB1X) | PcspB1, cspA signal peptide, xynA | This work |
| pXMJ19-xynA (P19-X) | pXMJ19 derivative, PcspB2, cspB2 signal peptide, xynA | This work |
| pEC-XK99E | E. coli–C. glutamicum shuttle vector, Kmr | Lab stock |
| pEC-XK99E-xynA (pEC-X) | pEC-XK99E derivative, PAH6, cspB2 signal peptide, xynA | This work |
| pK18mobSacB | SacB, suicide vector, Kmr | Lab stock |
| pK18mobSacB-cspB2 | pK18mobSacB derivative, for cspB2 disruption | This work |
| pK18mobSacB-xynA | pK18mobSacB derivative, for xynA integration | This work |