Figure 3.

Switch junctions with overlaps of eight nucleotides or more (all from PMS2-deficient mice). Overlap was determined by identifying the longest region at the switch junction of perfect uninterrupted donor/acceptor identity. Clone C2.39 (which is therefore determined as exhibiting an overlap of only five nucleotides) is nevertheless included as an example of a junction from a PMS2-deficient mouse (of a type not found in control mice), in which the donor/acceptor homology (highlighted in gray) extends beyond the longest region of perfect donor/acceptor identity. The germline Sμ sequence is not precisely known in the region further 3′ of that depicted for clone C2.42.