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Chromatograms of IQ mixture in plasma (A) and each isomer in methanol (B). (A) Top, double-blank plasma. Middle, plasma spiked with 500 ng/mL IQ mixture (333 ng/mL of IQ and 167 ng/mL of epi-IQ) and 100 ng/mL of the IS. Bottom, a real plasma sample containing 324 ng/mL of IQ and 58 ng/mL of epi-IQ. The monitored mass transitions for IQs and the IS were m/z 357.0→151.0 and 296.1→251.7, respectively.
