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. 2019 Mar 18;19(5):3497–3504. doi: 10.3892/mmr.2019.10050

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression, purification and IgE binding activity of rDer f 1/2. (A) Schematic diagram of the Der f 1/2 fusion protein construct. (B) SDS-PAGE of rDer f 1/2 following expression in Escherichia coli BL21(DE3) pLysS. (C) SDS-PAGE of rDer f 1/2 following purification by nickel affinity chromatography. Western blotting was conducted to determine the binding of rDer f 1/2 to specific IgE in the sera from (D) 15 house dust mite-allergic patients or (C) 15 non-allergic controls. 6×His-Tag, hexahistidine tag; Der f, allergen from Dermatophagoides farina; Ig, immunoglobulin; IPTG, isopropyl-β-D-thiogalactopyranoside; r, recombinant.