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. 2001 Nov 20;98(25):14559–14564. doi: 10.1073/pnas.251540598

Figure 1.

Figure 1

(A) Proliferation assay of ex vivo cultures of five HAM/TSP PBMC. The PBMC from five HAM/TSP patients (pt#) were cultured for 2 and 6 days with media alone or with addition of 5 μg/ml of various antibodies as indicated. Each assay was performed in triplicate. The average cpm from each of the patients was plotted with standard errors shown for each entry. (B) Tetramer staining of PBMC of five HAM/TSP patients (pt#) at day 0. The uncultured PBMC (day 0) were used in a FACS analysis using tetramer-Tax or tetramer-HIV and an antibody against the CD8 molecule. The cells were analyzed in FACS analysis in gates R1 and R2. At day 0, the majority (>95%) of CD8 cells were in R1. The percentage of cells in gated area R1 + R2, which reacted with tetramer-Tax and anti-CD8 antibody, was considered as Tax-specific CD8 cells. These numbers are indicated on the right as the percentage of the Tax-specific CD8 cells relative to the total CD8 cells.