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. 2001 Nov 20;98(25):14559–14564. doi: 10.1073/pnas.251540598

Figure 3.

Figure 3

The Ki-67 and Annexin V staining of HAM/TSP PBMC after 2- and 6-day cultures. The PBMC from HAM/TSP patients (pt#) were cultured for 2 or 6 days with media alone, anti-IL-2 + anti-Tac, or anti-IL-15 + Mikβ1. The cells were collected and stained with tetramer-Tax, anti-CD8 antibody, and either Ki-67 or Annexin V. The Tax-specific CD8 cells were gated and analyzed for Annexin V or Ki-67 staining. (A) The Ki-67 staining of Tax-specific CD8 cells in day 2 and day 6 is reported as the percentage of the Tax-specific CD8 cells that were positive for Ki-67 relative to the total Tax-specific CD8 cells. (B) The Annexin V staining of Tax-specific CD8 cells in day 2 and day 6 is reported as the percentage of the Tax-specific CD8 cells that were positive for Annexin V staining at day 2 or day 6 relative to the total number of Tax-specific CD8 cells.