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. 2001 Nov 20;98(25):14559–14564. doi: 10.1073/pnas.251540598

Figure 6.

Figure 6

Staining of the PBMC obtained from patient 2 with tetramer-CMV. (A) The uncultured PBMC from patient 2 were stained with tetramer-CMV or tetramer-HIV as a negative control as well as with an antibody against CD8 cells. The specific reactivity of tetramer-CMV with a population of CD8 cells identified the CMV-specific CD8 cells. (B) The PBMC were cultured for 2 or 6 days with media alone or with the addition of the various antibodies as indicated. The cells were stained with tetramer-CMV or tetramer-HIV as a negative control and an antibody against CD8 cells. The data are presented as the percentage of the CMV-specific CD8 cells relative to the total number of CD8 cells in both R1 and R2 gates. (C) The uncultured PBMC were examined for expression of IL-2Rs and IL-15Rs by CMV-specific CD8 cells. The cells were stained with tetramer-CMV, an anti-CD8 antibody, and antibodies against different chains of the IL-2R or IL-15R or an appropriate isotype control antibody. Two populations of CMV-specific CD8 cells and total CD8 cells were gated, and the expression of IL-2R and IL-15R by these cells was examined.