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. 2019 Mar 6;19(5):3584–3592. doi: 10.3892/mmr.2019.10012

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Transfection rate, transfected gene expression and culture conditions. (A) ISL-1-ADSCs observed at 48 h under a fluorescence microscope (magnification, ×40). (B) Tbx18-ADSCs observed at 48 h under a fluorescence microscope (magnification, ×40). (C) ISL-1+Tbx18-ADSCs observed at 48 h under a fluorescence microscope (magnification, ×40). (D) Transfection rate of different MOI values. (E) Expression of human ISL-1mRNA and Tbx18mRNA in the transfected groups. Scale bar=200 µm. *P<0.05 vs. GFP group. Tbx18, T-box 18; ISL-1, insulin gene enhancer binding protein 1; ADSCs, adipose tissue-derived stem cells; MOI, multiplicity of infection; GFP, green fluorescent protein.