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. 2019 Mar 6;19(5):3584–3592. doi: 10.3892/mmr.2019.10012

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Single-cell electrophysiology of ADSCs. (A) Spindle-shaped cells were used for all electrophysiological recordings (magnification, ×200). (B) Hyperpolarization-activated inward currents recorded from ISL-1-ADSCs using the patch clamp technique. (C) Hyperpolarization-activated inward currents recorded from Tbx18-ADSCs using the patch clamp technique. (D) Hyperpolarization-activated inward currents recorded from ISL-1+Tbx18-ADSCs using the patch clamp technique. (E) Hyperpolarization-activated inward currents (I_f) were blocked by CsCl (4 mmol/l). (F) Current density-voltage association of ISL-1 (red, n=6), Tbx18 (green, n=6) and ISL-1+Tbx18 (yellow, n=6) groups. Tbx18, T-box 18; ISL-1, insulin gene enhancer binding protein 1; ADSCs, adipose tissue-derived stem cells; I_f, funny current. Scale bar, 50 µm.