Table I.
Polymerase chain reaction primers used in this study.
| Gene | Primer sequences (5′-3′) | Product size (bp) |
|---|---|---|
| H-ISL-1 | 181 | |
| Forward | GCGGCAATCAGATTCACGAT | |
| Reverse | GCGCATTTGATCCCGTACAA | |
| H-Tbx18 | 173 | |
| Forward | TCCAAGGTACTGGGAATGGC | |
| Reverse | TGTGCTGTATCGGTTGAGGG | |
| R-HCN4 | 300 | |
| Forward | GCATCCACGACTACTACGAAC | |
| Reverse | TCTCCTTGTTGCCCTTAGTG | |
| R-cTnT | 202 | |
| Forward | GCAGGCTCTTCATGCCCAACT | |
| Reverse | CGCTCTGCCCGACGCTTTT | |
| R-Tbx3 | 168 | |
| Forward | TTACAGCCCGTATTCCATCCC | |
| Reverse | CGGCTATTCAGTTCCGACCC | |
| R-Nkx2.5 | 248 | |
| Forward | ACGCCCTTCTCAGTCAAAGA | |
| Reverse | TAAAATGTAGGGGCGGTTGG | |
| R-Cx43 | 242 | |
| Forward | GGCAAGGTGAAAATGAGGGG | |
| Reverse | AAAGCGAGAGACACCAAGGA | |
| R-Cx45 | 112 | |
| Forward | TTCTGATAATGTATGGTGTC | |
| Reverse | AGTTCCCTCCTTTTACTGTT | |
| R-GAPDH | 253 | |
| Forward | ACAGCAACAGGGTGGTGGAC | |
| Reverse | TTTGAGGGTGCAGCGAACTT |
H-ISL-1, human insulin gene enhancer binding protein 1; H-Tbx18, human T-box 18; R-HCN4, rat hyperpolarization-activated cyclic nucleotide-gated potassium channel 4; R-cTnT, rat cardiac troponin T; R-Tbx3, rat T-box 3; R-Nkx2.5, rat homeobox protein Nkx-2.5; R-Cx43, rat connexin 43; R-Cx45, rat connexin 45.