Table I.
A, Mimic and siRNA sequences | ||
---|---|---|
Name | Direction | Sequences |
miR-329-3p mimic | Sense | 5′-AACACACCCAGCUAACCUUUUU-3′ |
Antisense | 5′-AAAGGUUAGCUGGGUGUGUUUU-3′ | |
E2F1 siRNA | Sense | 5′-GCAGAAACGACGCAUCUAUTT-3′ |
Antisense | 5′-AUAGAUGCGUCGUUUCUGCTT-3′ | |
B, Reverse transcription-quantitative polymerase chain reaction primers | ||
Name | Direction | Sequences |
miR-329-3p | Forward | 5′-GCGCGAACACACCCAGCTAACCTTTTT-3′ |
U6 | Forward | 5′-CTCGCTTCGGCAGCACA-3′ |
Reverse | 5′-AACGCTTCACGAATTTGCGT-3′ | |
E2F1 | Forward | 5′-GAAGAAGACCGGTTGTCACC-3′ |
Reverse | 5′-GAAATCCAGAGGGGTCAAGTC −3′ | |
GAPDH | Forward | 5′-ATTCCATGGCACCGTCAAGGCTGA-3′ |
Reverse | 5′-TTCTCCATGGTGGTGAAGACGCCA-3′ |
miR, microRNA; si, small interfering; E2F1, transcription factor E2F1.