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. 2019 Mar 14;19(5):3649–3657. doi: 10.3892/mmr.2019.10027

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Copyright: © Fu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Overexpression of Creb protects PDLSCs from H₂O₂-induced cytotoxicity. (A) Reverse transcription-quantitative polymerase chain reaction and (B) western blot analysis illustrating Creb expression in PDLSCs transfected with pcDNA3.1 or pcDNA3.1-Creb for 48 h. (C) A total of 48 h post-transfection, PDLSCs were treated with 125 µM H₂O₂ for 24 h, and cell viability was detected by MTT assay. *P<0.05 vs. respective control. PDLSCs, periodontal ligament stem cells; Creb, cAMP responsive element binding protein.