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. 2019 Mar 26;19(5):4353–4363. doi: 10.3892/mmr.2019.10084

Figure 6.

Figure 6.

Inhibitors of TLR-4, p38 MAPK and NF-κB reduce the expression of TNF-α, IL-1β, and IL-6 mRNAs in peritoneal macrophages from C3H/HeN mice stimulated with anti-β2GPI–IgG. The peritoneal macrophages (2×106) from C3H/HeN mice (TLR-4 intact; n=8 in each group) were incubated with 5 µM TAK-242 (TLR-4 inhibitor) or 20 µM PDTC (NF-κB inhibitor) or 10 µM SB203508 (p38 MAPK inhibitor) for 2 h, then treated with NR-IgG (100 µg/ml), anti-β2GPI–IgG (100 µg/ml) or LPS (500 ng/ml) for 6 h. The total RNA were extracted from the macrophages and mRNA expression levels of (A) TNF-α, (B) IL-1β and (C) IL-6 were detected by reverse transcription-quantitative polymerase chain reaction. The data are representative of three separated experiments with the similar results. *P<0.05 vs. control NR-IgG; #P<0.05 vs. untreated. TLR-4, Toll-like receptor-4; TNF-α, tumor necrosis factor-α; PDTC, pyrrolidine dithiocarbamate; NR-IgG, isotype control antibody; Anti-β2GPI, anti-β2-glycoprotein I; LPS, lipopolysaccharide; IL, interleukin.