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. 2019 Mar 15;24(6):1032. doi: 10.3390/molecules24061032

Figure 2.

Figure 2

Electrophoresis representation of the Agilent Bioanalyzer 2100 gel-electropherograms of PCR products generated by primers flanking the internal transcribed spacer (ITS) region of ITS-1 and ITS-4 and PCR–restriction fragment length polymorphism (RFLP) products from G. simplicifolia. Lane 1, PCR product of the spacer region produced a band of about 758 bp; Lane 2, PCR–RFLP analysis using MspI G. simplicifolia digested PCR products gave three bands of about 471, 312, and 57 bp; Lane 3, PCR–RFLP analysis using HhaI G. simplicifolia digested PCR products produced five bands of about 319, 240, 215, 198, and 86 bp; Lane 4, PCR–RFLP analysis using HaeIII G. simplicifolia digested PCR products generated three bands of about 431, 198, and 86 bp. L = bp markers. The green bar indicates the lower marker (15 bp), whereas the violet band indicates the upper marker (1500 bp). See Supplementary data S1 for more information. The figure also shows the nucleotide sequences of the ITS gene spacer region of G. simplicifolia.