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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: Methods Mol Biol. 2018;1741:135–149. doi: 10.1007/978-1-4939-7659-1_11

Fig. 2.

Fig. 2

Quantitation of small amounts (ng to pg) of peptides using a MS-based assay. Standard amounts of a known mixture of peptides are analyzed by LC-MS/MS, and total matched peptide spectral intensities obtained for identified proteins are plotted against the amount analyzed as functions of log10. The resulting standard curve is then used for determining concentrations of unknown amounts of peptide pools generated from samples. AUC area under the curve