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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: Methods Mol Biol. 2018;1741:135–149. doi: 10.1007/978-1-4939-7659-1_11

Fig. 3.

Fig. 3

HILIC separation of 50 μg TMT-labeled peptide mixture. UV absorption at 214 nm (in mAU) is the “x” axis and elution time is the “y” axis. During the course of the chromatographic separation, a fraction is collected every 4 min, resulting in nearly 15 fractions of TMT peptide pools for subsequent total protein and phospho-peptide TiO2 enrichment analyses