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Medians of TMT ratios for mixing equal amounts of TMT labeled peptides
| Median ratios for each label | Channel IDs |
|---|---|
| 1.0 | Reporter intensity 126 |
| 1.0 | Reporter intensity 127N |
| 1.1 | Reporter intensity 127C |
| 1.0 | Reporter intensity 128N |
| 1.0 | Reporter intensity 128C |
| 0.9 | Reporter intensity 129N |
| 1.0 | Reporter intensity 129C |
| 1.0 | Reporter intensity 130N |
| 0.9 | Reporter intensity 130C |
| 0.9 | Reporter intensity 131 |
The mixing is optimized until the medians for each TMT channel are close to 1:1:1 etc.
