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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: J Thromb Haemost. 2018 Aug 13;16(10):2083–2096. doi: 10.1111/jth.14240

Fig. 1.

Fig. 1.

Gene targeting of Rac1 GTPase or small molecule inhibition of Rac1 diminished CRP-induced ROS generation in platelets. (A) CRP (0.5 μg mL−1) was added to washed human platelets and ROS was measured at the indicated time-points. CRP increased ROS generation in a time-dependent manner. (B) NSC23766, a Rac GTPase inhibitor [23], was added to platelets 2 min before stimulation with CRP (0.5 μg mL−1) and ROS was recorded at 10 min. (C) CRP (0.5 μg mL−1) was added to platelets from conditional Rac1 knockout mice [23] and ROS was measured at 10 min. Generation of ROS in dcf-da loaded platelets was monitored by flow cytometry as detailed in the Methods section. The data are mean ± SE, n = 4. *P < 0.0001, **P < 0.001. CRP, collagen-related peptide; MFI, mean fluorescence intensity; ROS, reactive oxygen species; SE, standard error.