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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: J Thromb Haemost. 2018 Aug 13;16(10):2083–2096. doi: 10.1111/jth.14240

Fig. 3.

Fig. 3.

Phox-I blocked thrombin-induced ATP secretion, platelet aggregation and rise in platelet cytosolic calcium. (A–C) Addition of thrombin to washed human platelets induced ATP secretion and platelet aggregation. A 2 min pre-incubation with Phox-I inhibited ATP secretion (mean ± SE, n = 4, *P < 0.0001) and platelet aggregation (n = 4, *P < 0.0001, **P < 0.001, ***P < 0.05) in a concentration-dependent manner. A Lumi-Aggregometer from Chrono-Log-Corporation was used to monitor platelet ATP secretion and aggregation. (D) Addition of thrombin to washed human platelets increased the cytosolic calcium level. Treatment of platelets with Phox-I 2 min before addition of thrombin inhibited the rise in calcium in a concentration-dependent manner. Changes in calcium levels were quantified in Fura2/AM loaded platelets as detailed in the methods section. [Color figure can be viewed at wileyonlinelibrary.com]