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. 2019 Apr 18;9:6270. doi: 10.1038/s41598-019-42746-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HG-treated HMCs secreted more exosomes than normal HMCs. Quantification of exosomes was normalized to the cell number by using the Countess^® Automated Cell Counter. The number of exosomes was quantified by using Nanoparticle Tracking Analysis. Six separate flasks were used, and the exosome number was quantified from three video recordings. Error bars are plotted as s.e.m. *p < 0.05 versus the HG-Exos 4 hr group, ^#p < 0.05 versus C-Exos. C-Exos group: exosomes derived from normal glucose-treated HMCs (Student’s t-test); HG-Exos group: exosomes derived from HG-treated HMCs.