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. 2019 Apr 18;9:6270. doi: 10.1038/s41598-019-42746-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exosomes characterization. (A) C-Exos. (B) HG-Exos. (C) C-Exos and (D) HG-Exos clusters with variable sizes. Images were obtained by transmission electron microscopy. Cup-shaped structures of 30–150 nm in size were identified as exosomes. Magnification: ×150,000. (E) Western blot analysis for exosome markers CD63 and CD81. (n = 3 for each index). (F) Histogram representing the profile of nanoparticle size by Nanoparticle Tracking Analysis. The values represent the means ± SEM, and all values are representative of at least three independent experiments.