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. 2019 Apr 18;9:6270. doi: 10.1038/s41598-019-42746-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Synthesis of AngII induced by C-Exos and HG-Exos in ACE-CHO. Chinese Hamster Ovary cells transfected with angiotensin converting-enzyme (ACE-CHO) were incubated with C-Exos or HG-Exos. After 24 hr, AngII staining was evaluated by immunofluorescence under confocal microscopy. For each 1 million ACE-CHO cells, 2 × 10⁸ particles/mL of C-Exos or HG-Exos was added in the presence and absence of captopril (ACE inhibitor). The staining for AngII (green) was not observed in ACE-CHO cells that were not treated with exosomes (control). The staining for AngII (green) was observed in ACE-CHO exposed to either C-Exos or HG-Exos. AngII staining was not observed in the presence of captopril. The images are representative of three independent experiments. Magnification of 630x. The nuclei were stained with DAPI (blue).