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. 2019 Apr 18;9:6293. doi: 10.1038/s41598-019-42627-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry. Collagen I, Fibronectin and Laminin are all present in their respective single protein scaffolds, as well as in varying degrees in the ECM scaffold, with no false positive staining observed in the control polymer only condition. Stains were performed for Collagen I, Laminin and Fibronectin,post processed using ImageJ. 10x magnification.