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. 2019 Apr 18;9:6293. doi: 10.1038/s41598-019-42627-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Q-PCR of key hepatic genes. Quantitative analysis of gene expression was undertaken on the functional cell layer at five, ten and sixteen days of culture, compared to that of the same culture periods grown on polymer only scaffolds. mRNA levels of Albumin (A), Cyp1A1 (B), Cyp1A2 (C), CYP3A4 (D), Collagen I (E), and Collagen IV (F) and Fibronectin (G) are represented as fold difference relative to polymer only controls and relative to the housekeeping gene GAPDH. One-way ANOVA with Games Howell and Tukey post hoc testing and minimum n = 5. *p < 0.05 **p < 0.01, ***p < 0.001. Error bars = SD.