Figure 2.

Infection dynamics of phage pVa-21 on biofilms of V. alginolyticus strain rm-8402. (a) Biofilms (48 h old) formed in 96-well plates were treated with pVa-21 at concentrations of 10⁷ PFU/mL. Total biofilm biomass was measured at OD₅₉₅ and stained with 1% crystal violet. Viable bacterial cell and phage counts were measured by direct plating on agar. The results represent the mean ± standard deviation of triplicate experiments. (b) Scanning electron micrograph of strain rm-8402 biofilm formation on glass coverslips. Scale bar = 10 μm and 2 μm at 2000× and 5000× magnification, respectively.