Figure 4.

The influence of LMWH and DOAC on tumour formation by AsPC-1 cells. Tumour spheroids were prepared by seeding out AsPC-1 cells (2 × 10⁴) in non-adherent Nunclon Sphera 96 wells plates in media (100 µl) containing a range of concentrations of Tinzaparin or Dalteparin (0–5 IU/ml), Apixaban (0–1 µg/ml) or Rivaroxaban (0–0.6 µg/ml). The cells were maintained at 37 °C, 5% CO₂ for 48 h to permit the form spheroid tumours. (A,B) The spheroid tumours were examined daily for up to 72 h, by white-light microscopy. (C) The rate of formation of intact tumours was then assessed from the size of the formed tumours and the number of detectable tumour particles. (n = 3, *p < 0.05 vs PBS control).