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. 2019 Apr 18;9:6272. doi: 10.1038/s41598-019-42738-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The influence of Tinzaparin and Becacizumab on vascularisation in CAM. The chorioallantoic membrane assay (CAM) were prepared and examined as described in the text. Gelfoam absorbable gelatine pads were soaked with a range of concentrations of Tinzaparin (0–5 IU/ml), Bevacizumab (0–12.5 µg/ml) or PBS control which were placed on the top of the CAM. (A) The extent of vascularisation within the CAM was then examined over 3 days and Images captured using Leica Application Suite software. (B) The vessel density and (C) average vessel diameter was determined in Tinzaparin-treated CAM, and was compared to the (D) vessel density and (E) average vessel diameter in Bevacizumab-treated samples. (n = 5, *p < 0.05 vs respective PBS control).