Table 5.
Felodipine plasma and brain concentration in mice during and after 28 days dosing via minipump
| Mouse (n) | Plasma (ng/ml) | Brain (ng/g) | |
|---|---|---|---|
| Day 10 | Day 28 | Day 28 | |
| 1 | 29.9 | 3.51 | <40a |
| 2 | 11.9 | 12.7 | 37.1 |
| 3 | 40.7 | 15.9 | 30.6 |
| 4 | 42.0 | 16.9 | 49.6 |
| 5 | 12.6 | 23.9 | <40a |
| 6 | 23.0 | 5.08 | <40a |
| 7 | 39.0 | 5.97 | 40.9 |
| 8 | 29.9 | 18.8 | 58.9 |
| Mean | 28.625 ± 4.23 | 12.845 ± 2.59 | 43.42 ± 3.9 |
Analysis was performed after 28 days of felodipine dosing of 5 mg/kg body weight/ day via osmotic minipump (Alzet Model 2002), flow rate 0.5 μl/h. Blood sample was collected at day 10 and day 28 (terminal) for analysis. Data is presented as actual values for individual mice and mean value +/− SEM shown in bold is provided in the final row. All osmotic minipumps in this study had no blockage, checked by remaining volume in the pump when replaced and at the end of the study
aindicates that samples which were below the limit of detection and were not included in the mean