Table 1.
A representative list of proteins from the iTRAQ data (mean ± standard error) showing a differential expression pattern with respect to ambient condition.
| Acc. ID | Locus tag | Protein name | 0.5%/0.04% CO2 | 1%/0.04% CO2 | 10%/0.04% CO2 |
|---|---|---|---|---|---|
| Inorganic carbon uptake and assimilation | |||||
| Q03513 | DOP62_10195 | Carbon dioxide concentrating mechanism protein CcmM | 0.09 ± 0.02 | 0.2 ± 0.05 | 0.07 ± 0.01 |
| Q03511 | DOP62_00505 | Carbon dioxide-concentrating mechanism protein CcmK | 0.11 ± 0.02 | 0.1 ± 0.04 | 0.13 ± 0.1 |
| Q31NB2 | DOP62_10175 | Ribulose 1,5-bisphosphate carboxylase small subunit | 0.03 ± 0 | 0.07 ± 0.01 | 0.09 ± 0.01 |
| Q9F1R2 | NA | HTH-type transcriptional activator CmpR | 0.48 ± 0.06 | NS | 0.12 ± 0.05 |
| Q55107 | DOP62_10270 | Bicarbonate transport ATP-binding protein CmpC | 0.08 ± 0.04 | 0.08 ± 0.03 | 0.04 ± 0.01 |
| P39660 | DOP62_10280 | Bicarbonate-binding protein CmpA | 0.02 ± 0 | 0.04 ± 0.01 | 0.02 ± 0 |
| Q8VPV7 | DOP62_05970 | CO2 hydration protein | 0.27 ± 0.06 | 0.32 ± 0.08 | 0.03 ± 0 |
| Q31ME6 | DOP62_10705 | NAD(P)H-quinone oxidoreductase subunit H | 0.29 ± 0.02 | 0.15 ± 0.01 | 0.06 ± 0.01 |
| Q31NJ5 | DOP62_07505 | NAD(P)H-quinone oxidoreductase subunit I | 0.11 ± 0 | 0.13 ± 0.04 | 0.05 ± 0.02 |
| Q31P07 | DOP62_12075 | NAD(P)H-quinone oxidoreductase subunit J | 0.18 ± 0.06 | 0.15 ± 0.05 | 0.08 ± 0.03 |
| Q31P08 | DOP62_12070 | NAD(P)H-quinone oxidoreductase subunit K | 0.43 ± 0.09 | 0.28 ± 0.1 | 0.05 ± 0.01 |
| Q31LQ7 | DOP62_07410 | NAD(P)H-quinone oxidoreductase subunit M | 0.15 ± 0.06 | 0.26 ± 0.04 | 0.14 ± 0.02 |
| Q31L05 | DOP62_04405 | NAD(P)H-quinone oxidoreductase subunit N | 0.29 ± 0.09 | 0.22 ± 0.03 | 0.07 ± 0.05 |
| Nitrogen absorption and transport | |||||
| P38043 | NA | Nitrate transport protein NrtA | 13.9 ± 5.15 | 11.41 ± 3.7 | 8.68 ± 3.08 |
| Q31PU9 | DOP62_11810 | Glutamate synthase (Ferredoxin) | 4.56 ± 1.54 | 3.8 ± 1.42 | NS |
| Q31L83 | NA | Glutamine synthetase | 11.8 ± 2.14 | 7.91 ± 1.61 | 6.17 ± 2 |
| P39661 | DOP62_10965 | Ferredoxin–nitrite reductase | 6.88 ± 2.72 | 7.11 ± 2.44 | 7.67 ± 2.63 |
| Photosynthesis and carbon fixation | |||||
| Q31LJ0 | DOP62_06080 | Photosystem I P700 chlorophyll a apoprotein A1 | 3.57 ± 0.72 | 4.52 ± 1.44 | 0.38 ± 0.05 |
| Q31LJ1 | DOP62_06085 | Photosystem I P700 chlorophyll a apoprotein A2 | 5.2 ± 0.56 | 8.3 ± 1.92 | NS |
| Q31PI7 | DOP62_06380 | Photosystem I reaction center subunit II | NS | 6.36 ± 2.36 | NS |
| Q31NT9 | DOP62_10920 | Photosystem I reaction center subunit III | 4.47 ± 1.37 | 5.91 ± 2.28 | NS |
| Q31NL7 | DOP62_08820 | Photosystem I reaction center subunit IV | 4.55 ± 2.48 | 8.9 ± 4.09 | 0.12 ± 0.03 |
| P95822 | DOP62_05055 | Photosystem I reaction center subunit XI | 5.9 ± 1.39 | 7.21 ± 1.56 | NS |
| Q31M07 | DOP62_09985 | Photosystem II 12 kDa extrinsic protein | NS | 3.75 ± 1.28 | NS |
| P11004 | DOP62_13040 | Photosystem II CP43 reaction center protein | 2.71 ± 0.51 | 2.28 ± 0.2 | 0.31 ± 0.06 |
| P31094 | DOP62_12825 | Photosystem II CP47 reaction center protein | NS | 1.81 ± 0.18 | 0.25 ± 0.08 |
| Q31RE4 | DOP62_00965 | Photosystem II lipoprotein Psb27 | 2.25 ± 0.19 | 3.73 ± 0.53 | 0.33 ± 0.11 |
| P11472 | DOP62_00570 | Photosystem II manganese-stabilizing polypeptide | NS | NS | 0.38 ± 0.09 |
| P04997 | DOP62_11795, DOP62_01605 | Photosystem II protein D1 2 | NS | 2.21 ± 0.15 | 0.07 ± 0.01 |
| Q31RG2 | DOP62_01060 | Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core | 14.39 ± 4.17 | 14.09 ± 4.39 | 5.42 ± 1.99 |
| Q31PD9 | DOP62_11780 | Phycobilisome rod linker polypeptide | 2.67 ± 0.44 | 2.93 ± 0.54 | NS |
| P55020 | DOP62_06590 | Plastocyanin | 10.27 ± 3.38 | 13.63 ± 1.63 | 9.09 ± 2.69 |
| Q8KPP3 | DOP62_12050 | Cytochrome b559 subunit alpha | 2.9 ± 0.35 | 3.13 ± 0.61 | 0.14 ± 0.04 |
| Q54711 | DOP62_05005 | Cytochrome b6 | 6.23 ± 2.53 | NS | NS |
| P25935 | DOP62_11225 | Cytochrome c6 | 19.64 ± 3.81 | 30.36 ± 5.91 | NS |
| P0A2Z8 | DOP62_04935 | ATP synthase epsilon chain | 11.66 ± 0.93 | 13.46 ± 1.27 | 12.98 ± 6.2 |
| Q31RF0 | DOP62_01000 | ATP synthase gamma chain | 4.61 ± 0.97 | 2.57 ± 0.22 | NS |
| Q31RF1 | DOP62_01005 | ATP synthase subunit alpha | 11.82 ± 1.87 | 8.03 ± 1.78 | 4.35 ± 1.91 |
| Q31RF3 | DOP62_01015 | ATP synthase subunit b | 5.53 ± 2.49 | 5.54 ± 1.59 | 3.75 ± 0.31 |
| Q31RF4 | DOP62_01020 | ATP synthase subunit b’ | 14.38 ± 3.66 | 12.09 ± 2.31 | 8.96 ± 2.19 |
| Q31KS4 | DOP62_04930 | ATP synthase subunit beta | 9.82 ± 1.5 | 6.4 ± 1.2 | 3.73 ± 0.76 |
| Q31RF5 | DOP62_01025 | ATP synthase subunit c | NS | NS | 6.5 ± 4 |
| Q31QY2 | DOP62_00835 | Sedoheptulose 1,7-bisphosphatase | 2.51 ± 0.4 | 2.87 ± 0.37 | NS |
| Q9R6W2 | DOP62_10710 | Glyceraldehyde-3-phosphate dehydrogenase | 5.79 ± 1.17 | 4.53 ± 0.68 | NS |
| Q31QU9 | DOP62_00660 | Transketolase | 5.31 ± 1.48 | 3.31 ± 0.85 | 2.58 ± 0.83 |
| Q935X4 | DOP62_03705 | Light-dependent NADPH-protochlorophyllide oxidoreductase | 7.73 ± 2.53 | 10.59 ± 2.72 | NS |
| Q31PM2 | DOP62_07035 | Porphobilinogen deaminase | 16.32 ± 5.01 | 20.42 ± 3.96 | 16.21 ± 2.13 |
| P16891 | DOP62_06580 | Uroporphyrinogen decarboxylase | 11.55 ± 1.97 | 8.03 ± 2.75 | 6.53 ± 1.38 |
| Q31QQ4 | DOP62_00020 | Mg-protoporphyrin IX chelatase | 9.03 ± 1.92 | 6.69 ± 1.66 | 5.4 ± 0.35 |
| Q9Z3G6 | DOP62_06830 | Heme oxygenase (Decyclizing) | 3.59 ± 0.88 | 3.53 ± 0.83 | 3.08 ± 0.65 |
| Q31LA2 | DOP62_05845 | Hydrogenobyrinic acid a,c-diamide cobaltochelatase | 7.13 ± 1.58 | 10.26 ± 1.48 | 5.26 ± 1.89 |
| Q31QJ2 | DOP62_13090 | Glutamate-1-semialdehyde 2,1-aminomutase | 10.02 ± 3.29 | 9.95 ± 2.3 | 6.56 ± 2.61 |
| Photoprotection and ROS response | |||||
| Q31M79 | DOP62_09835 | Flavoprotein | 0.25 ± 0.05 | 0.16 ± 0.04 | 0.15 ± 0.03 |
| Q31NJ4 | DOP62_07510 | NADH dehydrogenase subunit 6 | NS | 0.34 ± 0.16 | 0.18 ± 0.08 |
| Q31KG4 | DOP62_03465 | Chaperon-like protein for quinone binding in photosystem II | NS | 0.49 ± 0.02 | 0.33 ± 0.08 |
| Q7X4K8 | DOP62_04890 | Thioredoxin peroxidase | 0.27 ± 0.02 | 0.25 ± 0.11 | 0.24 ± 0.11 |
| Q31MK3 | DOP62_11435 | Thiosulphate-binding protein | 0.27 ± 0.03 | 0.18 ± 0.07 | 0.13 ± 0.04 |
| Q79PF2 | DOP62_09040 | Glutathione peroxidase | 0.26 ± 0.1 | NS | NS |
| Q31Q49 | DOP62_12620 | Glutathione S-transferase | 0.17 ± 0.06 | 0.2 ± 0.08 | 0.19 ± 0.08 |
| Q31QJ5 | DOP62_13105 | Bacterioferritin comigratory protein | 0.14 ± 0.07 | 0.35 ± 0.17 | 0.4 ± 0.14 |
| Q31L59 | DOP62_05365 | Bacterioferritin comigratory protein | 0.35 ± 0.15 | 0.37 ± 0.01 | NS |
| Glycolysis, TCA cycle and pentose phosphate pathway | |||||
| Q31R18 | DOP62_01165 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | 3.87 ± 0.67 | 5.65 ± 1.7 | 5.66 ± 0.96 |
| Q31NZ1 | DOP62_09015 | Dihydrolipoyl dehydrogenase | 3.45 ± 1.26 | 2.26 ± 0.33 | 4.06 ± 1.41 |
| Q31MH0 | DOP62_07935 | Isocitrate dehydrogenase [NADP] | NS | 0.25 ± 0.04 | 0.34 ± 0.1 |
| P21577 | DOP62_01965 | 6-phosphogluconate dehydrogenase, decarboxylating | 0.12 ± 0.03 | 0.08 ± 0.01 | 0.16 ± 0.02 |
| Q31KU2 | DOP62_04740 | Transaldolase | 0.25 ± 0.03 | 0.22 ± 0.03 | NS |
| Response to high CO 2 induced cellular stress | |||||
| P22880 | DOP62_04920 | 10 kDa chaperonin | 7.5 ± 3.65 | 10.31 ± 2.92 | 10.53 ± 1.3 |
| P22879 | DOP62_04915 | 60 kDa chaperonin | 4.93 ± 0.87 | 4.32 ± 0.75 | 5.25 ± 0.42 |
The accession IDs belong to the Synechococcus 7942 UniProt FASTA file. Additionally, the locus tags of Synechococcus 11801 proteins have been mentioned in the table. *NA = Not found, NS = Not significant.