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. 2019 Apr 18;10(5):336. doi: 10.1038/s41419-019-1569-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Scheme of NMN administration and downstream assays. b–c The percentage of fibroblast colonies that contained osteogenesis (b) and adipogenesis (c) differentiation of CFU-Fs are d qPCR analysis of expression for regulatory genes of osteogenic and adipogenic differentiation in LepR⁺CD45⁻Ter119⁻CD31⁻ cells from 5-month-old NMN-treated and control mice (n = 3 independent experiments). e–f Representative µCT images showing vertical (e) and cross (f) sections of whole femur. g–j µCT analyses of the cortical bone thickness (g), cortical bone area (h), total area (i), and cortical bone area/total area (j) in the femur metaphysis (n = 6 mice per group from six independent experiments). k Representative µCT images showing trabecular bone from the femur metaphysis. l–q µCT analyses of the percentage of trabecular bone volume out of total volume (l), trabecular number (m), trabecular thickness (n), trabecular spacing (o), connectivity density (p), and structure model index (q) in the femur metaphysis (n = 6 mice per group from six independent experiments). r–s Representative Oil Red S and hematoxylin staining in femur sections (r) and quantification of the adipocyte number (s) (n = 6 mice per group from six independent experiments). *p < 0.05, **p < 0.01 error bars, s.d.