Figure 2.

Identification of Cdc14 interactors using SILAC. (a) Experimental strategy. CDC14-/ MET3-cdc14^PD–Myc cells were grown in depressing heavy medium alongside parental untagged cultures grown in light medium and the lysates mixed in equal quantities prior to mass spectrometry analysis. Cdc14^PD–Myc was immunoprecipitated and the immuneprecipitate analysed using a quadrupole Orbitrap-MS. Peptides from genuine interacting proteins will show a significant deviation from 1:1 H:L ratio. (b) Volcano plot of intensity versus H:L ratio of detected proteins. Blue dots show proteins enriched in heavy peptides in both yeast and hyphae, yellow dots show proteins only enriched in heavy peptides in the yeast form and grey dots indicate proteins that show no heavy peptide enrichment. Proteins detected in the yeast IPs or hyphal IPs but not detected in the respective lysates are listed in Supplementary Dataset S2.