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. 2019 Apr 18;9:6278. doi: 10.1038/s41598-019-42179-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Absence of maternal DCAF13 abrogates zygotic nucleus function in transcription and results in decreased translation. (a,b) Graphical representation of the mean expression level ± standard error of the mean (SEM) by reverse transcription polymerase chain reaction (RT-PCR) for several ribosomal protein genes (a) and rDNA processing factor gene (b) in Dcaf13^♀+/♂+ (in gray) or Dcaf13^♀−/♂+ (in red) zygotes, two-cell and four-cell (two-cell stage in Dcaf13^♀−/♂+) embryos. *p < 0.05, ** to p < 0.01 and*** to p < 0.001. (c) Immunofluorescence using antibodies against H4K16Ac (in green) and DNA (in red) in two-cell embryos (top), and intensity measurement (bottom). Scale bar, 20 µm. *** to p < 0.001. Dcaf13^♀+/♂+ (n = 16), Dcaf13^♀−/♂+ (n = 16). (d) Detection of newly synthesized protein by homopropargylglycine (HPG) incorporation in two-cell embryos of Dcaf13^♀+/♂+ or Dcaf13^♀−/♂+ was shown (top), also with the intensity measurement (bottom). Scale bar, 20 µm. *** to p < 0.001. Dcaf13^♀+/♂+ (n = 14), Dcaf13^♀−/♂+ (n = 14). (e) Graphical representation of the mean expression level ± SEM by RT-PCR for several representative zygotic genes in Dcaf13^♀+/♂+ (in gray) or Dcaf13^♀−/♂+ (in red) zygotes, two-cell and four-cell (two-cell stage in Dcaf13^♀−/♂+) embryos. *p < 0.05, ** to p < 0.01, and *** to p < 0.001.