Fig. 2.

ZFP30 regulates multiple targets during adipogenesis. a, d Number of genes significantly (FDR 0.1, |FC| 2) down- (a) or up- (d) regulated upon Zfp30 KD in 3T3-L1 cells at days 0 and 2 upon adipogenesis (left) as well as Zfp30 KO in IBA cells at days 0, days 2, and 4 upon adipogenesis (right) (light gray). The number of commonly deregulated genes is depicted in dark gray. b,e Gene Ontology (GO) biological processes enriched among the genes commonly down- (b) and up- (e) regulated upon Zfp30 KD and KO at days 2 or 4. c, f Heatmap displaying the top 30 genes commonly down- (c) and up- (f) regulated upon Zfp30 KD and KO at days 2 or 4, ordered based on FCs in day 2 KD samples. g Number of ZFP30-HA ChIP-seq peaks in 3T3-L1 cells at days 0 and 2. Two biological replicates (R1 and R2) were performed. h The overlap between ZFP30-HA ChIP-seq peaks at days 0 and 2. i Examples of ZFP30-HA DNA binding at the Oas1 gene cluster (adipogenic-invariant binding) and Glis3 locus (adipogenesis-induced binding). j DNase I hypersensitivity (upper panel) and H3K4me1 (lower panel) signal upon adipogenic induction in a 4 kb window centered around the point of maximal ZFP30 binding, at locations bound by ZFP30 at day 2 only. k Top de novo motif (M1) discovered in DNA sequences bound by ZFP30 in mouse 3T3-L1 cells. l In vitro ZFP30 binding to DNA oligos bearing the consensus M1 motif as well as a control sequence, as assessed using MITOMI. The quantities of bound DNA are expressed in relative fluorescent units (RFU), normalized by protein amount. n = 3 biologically independent experiments. **p < 0.01, t test. m Top de novo motif discovered in DNA sequences bound by ZFP30 in human HEK239T cells. All panels: error bars—SD. Source data are provided as a Source Data file