Figure 4.

Effect of ibuprofen on the incorporation of [¹⁴C]acetyl aspirin radioactivity into microsomal platelet proteins. Washed platelets were treated with vehicle (○) or 10 μM ibuprofen (□) for 5 min before the addition of 100 μM [¹⁴C]acetyl aspirin. After 30 min, the cells were washed and lysed, and the microsomal fraction was solubilized with octylglucoside before electrophoresis on denaturing gels. Horizontal fractions were excised and counted in scintillation fluid. Fraction 4 represents the beginning of the separating gel. The mobility of molecular mass markers (in kDa) are shown by arrows. The data shown are representative of multiple results obtained with ibuprofen (n = 2) and vehicle-treated platelets (n = 4).