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. 2019 Apr 18;10(5):341. doi: 10.1038/s41419-019-1572-7

Fig. 6. Effects of baicalein on fibronectin (FN), calpain-2, E-cadherin, and vimentin expression in breast tissues of MMTV-PyMT mice.

Fig. 6

a Protein extracts from tumors were analyzed by western blotting with FN, calpain-2, E-cadherin, and vimentin antibodies. Protein levels were quantified and normalized to GAPDH. The results are shown as fold-change compared with control (Ctrl) cells. Data are presented as mean ± SEM for three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001. b Representative images of tumor sections immunostained with anti-mouse-FN, anti-mouse-calpain-2, anti-mouse-E-cadherin, or anti-mouse-vimentin antibodies (×400). Scale bars: 50 μm. c FN expression correlated with survival of breast cancer patients. Kaplan–Meier survival curves suggested that high FN expression was associated with poor relapse-free survival (RFS, n = 3951), distant metastasis-free survival (DMFS, n = 1746), and palliative performance scale (PPS, n = 414) compared with lower FN expression