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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: Exp Hematol. 2018 Oct 10;69:37–42. doi: 10.1016/j.exphem.2018.10.001

Figure 2. Menin inhibitors phenocopy Men1 deletion and collaborate with Mll1;Mll2 loss to kill leukemia cells.

Figure 2.

A) GSEA plots showing enrichment of MI-2–2 regulated genes20 in Men1-deleted MLL-AF9 cells and in Mll1;Mll2-deleted MLL-AF9 cells. B) qRT-PCR of selected target genes after MI-2–2 treatment in MLL-AF9-transformed cells. Gene expression was determined at 72 hours post treatment. Data are represented as averages ± SD. C-D) Relative cell viability after and MI-2–2 (C) and MI-2 (D) treatment in MLL-AF9-transformed cells with or without Mll1;Mll2. MLL-AF9-transformed cells with the genotype of Cre+; Mll1F/F; Mll2F/F were treated with ethanol or 4-OHT for 24 hours, washed, then treated with compound or vehicle for 3 additional days. Cell viabilities were all normalized to corresponding vehicle (ethanol) control. Data are represented as mean ± SD. One representative of two independent experiments is shown.