Fig 5. [³H]-Cholesterol efflux from BAC/APOL1 mouse bone marrow-derived macrophages.

Cultured macrophages were polarized into M1 macrophages with IFN-γ (20 ng/mL) and M2 macrophages with IL-4 (20 ng/mL). Following labeling macrophages with [³H]-cholesterol during 18 h, each [³H]-cholesterol efflux into media was induced by 4 h-incubation with the mouse serum as a cholesterol acceptor. Cholesterol efflux was reduced in APOL1-G1 and APOL1-G2 macrophages compared to APOL1-G0 macrophages;.this was true for both M1 and M2 macrophages. The results are representative of independent experiment (N = 6 mice per group, 3–5 samples per mouse; median is shown). One-way ANOVA test; **P < 0.01, ***P < 0.001.