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. 2019 Apr 18;14(4):e0215298. doi: 10.1371/journal.pone.0215298

Fig 2. MBP-Pro45-70-His6 blocks MSTN-induced Smad2/3 phosphorylation in HepG2 cell.

Fig 2

HepG2 cells were cultured in serum-free DMEM for 4 h, followed by treatment with the combination of 10 nM MSTN, 10 μM SB431542, or 600 nM MBP-Pro45-70-His6 for 30 min. The blots are representative of three independent assays and have been sequentially probed with antibodies against phospho-Smad2 (p-Smad2) or phospho-Smad3 (p-Smad3), total Smad2 (Smad2) or total Smad3 (Smad3), and b-actin. Densitometry analyses of the blots were from three independent assays. The Relative phosphorylation levels of p-Smad2 and p-Smad3 were normalized by the expression level of total Smad2 (A) or total Smad3 (B), respectively. The error bars indicate standard deviation (n = 3). Different letters are significantly different at P<0.05.